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نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Characterization of Mucor racemosus lipase with potential application for the treatment of cellulite
Characterization of Mucor racemosus lipase with potential application for the treatment of cellulite
 
الموضوع : كيمياء حيوية 
لغة الوثيقة : الانجليزية 
المستخلص : An extracellular lipase LII from Mucor racemosus was purified by anion exchange and gel filtration chromatography using DEAE-Sepharose and Sephacryl S-200 columns, respectively. A molecular weight of 20 kDa was estimated for M. racemosus LII by Sephacryl S-200 column and SDS-PAGE. LII had an acidic pI at 4.8. Vmax and Kmfor LII were estimated to be 55.5 μmol oleic acid/min/ml and 2% olive oil, respectively. The highest lipolytic activity was detected with linseed oil as the substrate. The optimal pH and temperature for LII was 5.0 and 40 °C, respectively. The enzyme was stable up to 70 °C. The lipase activity was strongly enhanced by Cu2+, Al3+ and Fe3+, and slightly enhanced by Mg2+, Ni2+ and Mn2+, whereas Ca2+, Co2+, K+ and Hg2+ showed no effect. Pb2+ only caused a partial inhibition of the enzyme. The residual lipase activity in carbopol 934 base gel was 385% after storage at 4 °C for 420 days. The characteristic properties of M. racemsus lipase formulated in the carbopol 934 gel was utilized for topical treatment of cellulite, and the results were positive for reducing thigh circumference. 
ردمد : 1359-5113 
اسم الدورية : Process Biochemistry 
المجلد : 46 
العدد : 3 
سنة النشر : 1432 هـ
2011 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Saturday, February 18, 2012 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
صالح أحمد محمدMohamed, Saleh Ahmedباحثدكتوراهsaleh38@hotmail.com
Heidi M Abdel-MageedAbdel-Mageed, Heidi Mباحثدكتوراه 
Saadia A TayelTayel, Saadia Aباحثدكتوراه 
Mohamed A El-NabrawiEl-Nabrawi, Mohamed Aباحثدكتوراه 
Afaf S FahmyFahmy, Afaf Sباحثدكتوراه 

الملفات

اسم الملفالنوعالوصف
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