تفاصيل الوثيقة
|
نوع الوثيقة |
: |
مقال في مجلة دورية |
|
عنوان الوثيقة |
: |
Ficus sycomorus latex: A thermostable peroxidase Ficus sycomorus latex: A thermostable peroxidase |
|
الموضوع |
: |
كيمياء حيوية |
|
لغة الوثيقة |
: |
الانجليزية |
|
المستخلص |
: |
Peroxidase from sycamore fig Ficus sycomorus latex (POLI) was purified by heat treatment, anion exchange chromatography and molecular exclusion chromatography. The purity was determined from high specific activity (9166 units/mg protein), purification fold (28), RZ value 3.1 and a single band in native polyacrylamide gel electrophoresis (PAGE), sodium dodecyl sulfate (SDS)-PAGE and visualized peroxidase activity on the PAGE. POLI had molecular mass of 43 kDa. Substrates commonly used in immunodiagnostic kits as 2,2-azino-bis [3-ethyl-benzothiazoline-(6)-sulfonic acid] (ABTS), 4-chloro-1- naphthol (4C-1N), o-phenylenediamine (OPD) and 3,3',5,5'- tetramethylbenzidine (TMB) were found to be the best substrates for the enzyme. The Km for catalysis of H2O2 was 1.2 mM. The catalytic efficiency (Vmax/Km) for POLI was found to follow the order: ABTS, 4C-1N, OPD, TMB, guaiacol, paminoantipyrine, o-dianisidine and pyrogallol. The enzyme showed a broad pH optimum ranged from pH 5.5 to 7.0. The optimal temperature for the enzyme was 35 to 40°C. POLI showed highest thermal stability. No loss of enzyme activity was recorded up to 60°C, whereas only 20% of enzyme activity was lost at 70 to 90°C. The thermal inactivation profiles of POLI demonstrated that the enzyme had higher thermal resistance. The peroxidase activity was slightly enhanced by low concentration of Ca2+, Ni+2 and Mg2+ and high concentration of Mn2+. Fe+3, Zn+2, Hg+2 caused slightly inhibitory effects. In conclusion, sycamore fig latex will be a new and potential source for a peroxidase enzyme. |
|
ردمد |
: |
1684-5315 |
|
اسم الدورية |
: |
African Journal of Biotechnology |
|
المجلد |
: |
10 |
|
العدد |
: |
76 |
|
سنة النشر |
: |
1432 هـ
2011 م |
|
نوع المقالة |
: |
مقالة علمية |
|
تاريخ الاضافة على الموقع |
: |
Saturday, February 18, 2012 |
|
الباحثون
| صالح أحمد محمد | Mohamed, Saleh Ahmed | باحث | دكتوراه | saleh38@hotmail.com |
| Azza M Abdel-Aty | Abdel-Aty, Azza M | باحث | دكتوراه | |
| Mohamed Belal Hamed | Hamed, Mohamed Belal | باحث | دكتوراه | |
| Mohamed O El-Badry | El-Badry, Mohamed O | باحث | دكتوراه | |
| Afaf S Fahmy | Fahmy, Afaf S | باحث | دكتوراه | |
|
الرجوع إلى صفحة الأبحاث
|