Document Details

Document Type : Thesis 
Document Title :
Assaying single and combined mutagenic effects of green tea and Rhazya stricta leaves extract in mice
تحليل التأثير المفرد والمشترك لمستخلص أوراق نبات الحرمل والشاي الأخضر
 
Subject : biological sciences department 
Document Language : Arabic 
Abstract : Rhazya stricta is a medicinal plant used in a folk medicine to treat many diseases in the Middle East especially in Saudi Arabia. Recent studies focused on about the genetic role and anti-cancer effect of this plant. The aim of this study was to examine whether the Rhazya leave aqueous extract have a mutagenic activity or not, in addition to the examination of the potential avoidance of this effect by green tea using cytogenetic and molecular assays in mouse. In the present study, Rhazya leaves aqueous extract showed a significant mutagenic effect at the cytogenetic level. The results of the micronucleus test showed that, there are a significant increasing in the formation of micronuclei in reticulocytes blood cells using the three different concentrations of Rhazya leave aqueous extract (0.23, 0.36, 0.47) gm/kg, while there was a decrease in the formation of micronuclei in reticulocytes blood cells in all combined treatments (green Tea, 3% + the three mentioned Rhazya concentrations). At the molecular level, seven random primers were used for the identification of RAPD-PCR markers. The primers amplified 75 bands, 50 of them were polymorphic about (66.7 %), 13 of them were monomorphic (17.33%) and 12 of them were unique (16%). The obtained RAPD patterns from the treated groups showed some differences with to control group with all the used RAPD primers. While the Observed patterns in the combined treated groups of green Tea and Rhazya leaves aqueous extract have almost the same banding pattern of green Tea treatment and control. All these results strongly point out to “under the test condition, Rhazya leaves aqueous extract has a mutagenic effects and green Tea has a positive role of in reducing this effect”. 
Supervisor : Dr. Saleha Yehya Alakilli 
Thesis Type : Master Thesis 
Publishing Year : 1434 AH
2013 AD 
Co-Supervisor : Dr. Hassan Mamdouh El Ashmaoui 
Added Date : Thursday, December 5, 2013 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
سلمى خالد بديويBedaiwi, Salma KhalidResearcherMaster 

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